65°c water bath (Thermo Fisher)

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Thermo Fisher 65°c water bath
65°C Water Bath, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%E2%97%A6+c/10__22175_slash_mmb__17751-107-11-13
Average 90 stars, based on 1 article reviews

65°c water bath - by Bioz Stars, 2026-09

90/100 stars

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Cell Culture:

Article Title: Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Loaded with Emodin Alleviate Intestinal Injury in Acute Pancreatitis.
Article Snippet: The cell suspension was centrifuged at 1200 rpm for 3 min (Eppendorf, TSGP10). .. CCD-841CON cells were resuspended in DMEM/F12 medium supplemented with 10% foetal bovine serum (ExCell Bio, Cat. FCS500) and 1% penicillinstreptomycin (ThermoFisher, Cat. 15070063) in DMEM/F12 medium, and cultured in a 6 cm culture dish (Corning, 354401) at 37◦C under 5% CO2 (ThermoFisher, 3311E). hUC-MSCs were cultured in the vendor-supplied dedicatedmedium (IMMOCELL, Cat. IMP-H054-1). .. For subculture, confluent cells were washed with phosphate buffer solution (PBS, Corning, Cat. 21-040-CV), digested with 0.25% trypsin (IMMOCELL, Cat. IMC-305) at 37◦C until detachment, and neutralized with complete medium.

Incubation:

Article Title: Tumor-specific glyco-detonators: A neuraminidase-3-gated programmable DNA Nanoarchitectonics for liver cancer-exclusive chemotherapy.
Article Snippet: The silent progression and lack of reliable biomarkers for early-stage hepatocellular carcinoma (HCC) often lead to late-stage diagnoses, where conventional chemotherapies are highly toxic and offer limited efficacy.. To bridge this diagnostic-therapeutic gap, we designed a DNA nanocomputer that functions as a biological logic gate, converting a pathological marker into a therapeutic command.. This “dual-lock” system uses sialic acid ‘caps’ to mask galactose ligands on a DNA scaffold.

Article Title: Carbamate-based cannabinoids uncover a conserved lipid-sensing pocket in nociceptive TRPA1 and TRPV1 channels.
Article Snippet: .. Briefly, transfected rTRPV1, rTRPV2, hTRPV1 or hTRPA1 HEK293T cells (wt or mutated); rTRPV1 or hTRPA1 Flp-In TREx 293 stable cells were spotted at Poly-D-Lysine (PDL, 0.1 mg/mL) coated imaging chambers (μ-slide, 8 well) and incubated for 30–40 min in a humidified incubator at 37◦C and 5% CO2 before being loaded with 5 μM Fura− 2 AM (Invitrogen; cat. no. F1201) dissolved in Ringer's solution (in mM: 140 NaCl, 2.5 KCl, 1.8 CaCl2, 2 MgSO4, 20 HEPES and 5 D-glucose, pH 7.4 with NaOH) supplemented with Pluronic F− 127 acid (Sigma-Aldrich; 0.01%) and incubated for 1 hr in the dark at RT. ..

Article Title: De novo pyrimidine synthesis controls germinal center B cell and plasma cell fates and systemic autoimmunity.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PerCP/Cy5.5 Conjugation Kit – Lightning Link AbCam Cat# ab102911 Alexa Fluor 647 Conjugation Kit (Fast) – Lightning Link AbCam Cat# ab269823 CellTrace Yellow Cell Proliferation kit, for flow cytometry Invitrogen Cat# C34573 Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit Thermo Fisher Scientific Cat# C10425 EasySep Mouse B cell Isolation Kit StemCell Technologies Cat# 19854 Seahorse XFe96/XF Pro FluxPak Agilent Technologies Cat# 103792-100 Immulon 4 HBX 96-well plates Thermo Scientific Cat# 3855 Multiscreen hydrophobic PVDF plates Millipore Sigma Cat# MSIPS4W10 Hep–2 slides Antibodies Incorporated Cat# 15-112 High-capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4374966 ADP/ATP Ratio Assay Kit Sigma Aldrich Cat# MAK135 nCounter Mouse Metabolic Pathways Panel nanoString Technologies Cat# XT-CSO-MMP1-12 Deposited data RNA-sequencing Raw and processed data This paper GEO: GSE329002 Metabolomics and isotope tracing Raw data This paper Metabolomics Workbench: Project ID PR003059 https://doi.org/10.21228/M88G4 Experimental models: Organisms/strains Mouse: B6: C57BL/6 Jackson Laboratories Strain: 000664; RRID: IMSR_JAX:000664 Mouse: FcγRIIB− /− : B6;129 S-Fcgr2btm1Ttk/J Jackson Laboratories Strain: 002848; RRID: IMSR_JAX:002848 Mouse: B6.yaa: B6.SB-Yaa-J Jackson Laboratories Strain: 000483; RRID: IMSR_JAX:000483 Mouse: UMPSfl: C57BL/6 J-Umpsem1Cflox/Cya Cyagen Cat# S-CKO_06542; Strain ID: CKOCMP-22247- Umps-B6J-VA Mouse: CD23Cre+: B6.Cg-Tg(Fcer2a-cre)5Mbu Jackson Laboratories Strain: 028197; RRID: IMSR_JAX:028197 Software and algorithms FlowJo v9 and v10 Waters Biosciences http://www.flowjo.com Seahorse Wave Pro Agilent Technologies N/A Agilent Seahorse Analytics Agilent Technologies N/A Perseus Tyanova et al.63 N/A nSolver 4.0 Analysis Software nanoString Technologies N/A Leica Application Suite (LAS) X software Leica Microsystems N/A NovaSeq Control Software (NCS) Illumina N/A Illumina bcl2fastq Conversion Software v2.20 Illumina N/A GeneSCF Subhash and Kanduri64 N/A Panther Classification System Gene Ontology Paul Thomas Lab https://pantherdb.org Cell Reports 45, 117587, July 28, 2026 23 figure legend. .. B cells were incubated at 37◦C for 24 h, then 6-azauridine was added at the indicated concentrations and cells were incubated for another 72 h. To detect EdU by flow cytometry, cells were surface stained, then Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit was used according to manufacturer’s protocol (ThermoFisher Scientific). .. Stained cells were analyzed on a BD FACSymphony A5 Cell Analyzer (BD Biosciences).

Article Title: Sex-specific acceleration of Alzheimer's pathogenesis by chronic sleep-deprivation.
Article Snippet: .. Following three 5-min TBS-T washes, membranes were incubated overnight at 4◦C with primary antibodies described in Table S2 in SuperBlock/TBS solution: ATG5 (Invitrogen, Catalog No.: PA1-46178), Beclin-1 (Invitrogen, Catalog No.: 66665-1-Ig), GAPDH (Millipore Sigma, CatalogNo. .. : G8795), LC3B (Cell Signaling Technology, Catalog No.: 2775S), and PHF1 (Albert Einstein College of Medicine, Catalog No.: AB_2315150).

Concentration Assay:

Conjugation Assay:

Liquid Chromatography with Mass Spectroscopy:

Mass Spectrometry:

Transfection:

Imaging:

Polymerase Chain Reaction:

Article Title: Germline sequence variation within the ribosomal DNA is associated with human complex traits.
Article Snippet: .. Preparation of LCL cell line lysate was adapted from a public protocol for polysome profiling followed by quantitative PCR.64 A minimum of 50 million LCLs were treated with 100μg/ml cycloheximide (Thermo Fisher Scientific Cat. No. J66004.XF) for 15 min at 37◦C. .. Cells were pelleted by centrifugation at 500g for 3 min at 4◦C, and washed twice with ice-cold PBS (Thermo Fisher Scientific Cat. No. 10010023) supplemented with 100μg/ml cycloheximide.

Flow Cytometry:

Staining:

Article Title: Bioactive fibrous microwell platforms induce m1 macrophage clusters that enhance anti-tumor activity and T cell polarization
Article Snippet: Engineering immune-regulatory microenvironments that direct macrophage function is central to advancing immunotherapies for solid tumors.. Here, we present a bioactive fibrous microwell platform that integrates electrospun polycaprolactone/polyvinylpyrrolidone (PCL/PVP) nanofibers with photopatterned threedimensional confinement and sustained lipopolysaccharide (LPS) delivery, enabling the formation of stable M1-polarized macrophage clusters beyond conventional transient stimulation.. The hybrid microenvironment guided macrophages into compact clusters while providing continuous pro-inflammatory cues.

Suspension:

Article Title: ZBTB21 Is a Dual Suppressor of Pyroptosis and MHC-I Antigen Presentation That Promotes Tumor Immune Evasion.
Article Snippet: .. The tissue fragments were resuspended in 10 mL of Hank’s Solution containing 2 mg/mL collagenase I (Gibco, Cat#17100017) and digested at 37◦C with shaking at 140 rpm for 50 min. Digestion was terminated by adding an equal volume of complete medium, and the resulting cell suspension was filtered through a 40 μm cell strainer into a new 50 mL centrifuge tube. .. After centrifugation at 1800 rpm for 5 min, the supernatant was removed, and the pellet was resuspended in 1 mL of erythrocyte lysis buffer (Solarbio, Cat#R1010) for 10 min on ice to lyse red blood cells.

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